Detailed information

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Project title:
Skeletal muscle structural and functional adaptations to training- versus immobilization-induced sarcomerogenesis in rats
Principal investigator(s):
Hinks, Avery
Co-investigator(s):
N/A
Supervisors:
N/A
Institution paid:
University of Guelph
Research institution:
University of Guelph
Department:
Human Health and Nutritional Sciences
Program:
Master's Award: Canada Graduate Scholarships
Competition (year/month):
202012
Assigned peer review committee:
Special Cases - Awards Programs
Primary institute:
N/A
Primary theme:
N/A
Term (yrs/mths):
1 yr 0 mth
CIHR contribution:
Contributors:
Amount:
$17,500
Equipment:
$0
External funding partner(s):
Partner Name:
N/A
Amount:
N/A
Equipment:
N/A
External applicant partner(s):
Partner Name:
N/A
Amount:
N/A
Equipment:
N/A
External in-kind partner(s):
Partner Name:
N/A
Amount:
N/A
Equipment:
N/A
Keywords:
Collagen; Crosslinking; Eccentric Training; Immobilization; Muscle Architecture; Muscle Stretch; Rat Soleus; Sarcomerogenesis; Work Loops
Abstract/Summary:
Immobilization of a muscle at a long length and exercise training biased to eccentric contractions both induce hypertrophy by addition of sarcomeres in series. However, less is know about how these experimental interventions influence intramuscular connective tissue, which contributes to force transmission and mechanical efficiency during locomotion. Existing research suggests eccentric training increases collagen content in intramuscular connective tissue while immobilization at a long muscle length results in no change, and there has been little investigation into collagen quality (i.e. collagen fibril crosslinking parameters). Using Sprague-Dawley rats, the present study will investigate adaptations in soleus muscle architecture and intramuscular connective tissue - with attention to both collagen content and crosslinking - following 4 weeks of immobilization of the ankle in a fully dorsiflexed position or eccentric-biased training induced by weighted downhill running. Additionally, work loop performance will be tested on the soleus in vitro to investigate the impact of differential collagen adaptations with increased serial sarcomere number on mechanical efficiency during locomotion.
Version:
20250311.1