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Project Information
Project Title:  Mechanism of type IV secretion encoded by the F plasmid
Period:  from: 2003-04-01 to: 2006-03-31  
Principal Investigator(s): Frost, Laura Susan ; Hazes, Bart  
Co-Investigators:  
Supervisors:  
Previous Investigators/Supervisors:  
Institution: University of Alberta  
Department: Biological Sciences 
Program: Operating Grant 
Assigned PRC: MI 
Primary Institute: Infection and Immunity 
Primary Theme: Biomedical 
Keywords: BACTERIAL CONJUGATION, BIOLOGIE MOLÉCULAIRE, GASTROINTESTINAL TRACT INFECTIONS, INFECTIEUSES ET PARASITAIRES, INFECTIOUS AND PARASITIC, MOLECULAR BIOLOGY, PROTEIN STRUCTURE AND FUNCTION, PROTEIN-PROTEIN INTERACTIONS, TYPE IV SECRETION, UROGENITAL TRACT INFECTIONS 
Abstract: F plasmid-mediated DNA transfer between bacteria has been recently grouped within the Type IV secretion complex which includes the transport of DNA, proteins and nucleoprotein complexes. Genome analysis has revealed the presence of potential Type IV-like systems within virulence plasmids, pathogenicity islands and the genomes of many pathogenic. The F transport apparatus consists of 15 proteins which form a structure that spans the cell envelope and is involved in the assembly of an extracellular filament, the F-pilus. The pilus, together with TraG and TraN, the mating pair stabilization proteins, have been implicated in identifying suitable recipient cells, signaling that conjugative DNA synthesis should begin and actual DNA transport. Based on preliminary genetic and biochemical evidence, we propose to study the construction of the conjugative pore using molecular biology and structure determination techniques. This application will focus on the proteins (TraB,F,G,U) in the periplasm and inner membrane as well as other selected proteins associated with them. These studies will provide insight into the mechanism of horizontal gene transfer, a process that is widespread in nature, with the goal of targeting type IV systems for potential therapeutic and biotechnological uses. 

Funding Information
Fiscal Year Amount
2002-03 $13,776
2003-04 $55,104
2004-05 $68,880
2005-06 $68,880
Total: $206,640